Eosin Methylene Blue Agar. Eosin Methylene Blue Agar Levine is used for the isolation and differentiation of Gramnegative enteric bacilli and is not intended for use in the diagnosis of disease or other conditions in humans This medium was introduced in 1916 by HoltHarris and Teague to differentiate Escherichia spp and Aerobacter spp.
It is used frequently in clinical laboratories Agents of EMB The selective/ inhibitory agents of EMB are the dyes eosin Y and methylene blue Methylene blue inhibits the gram + bacteria (eosin to a lesser extent) while eosin changes color to a dark purple when the medium around the colony becomes acidic EMB contents.
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Eosin methylene blue (EMB) and Tergitol7 agar are alternative lowselectivity agar containing lactose Colourless colonies on EMB plates or bluish colonies on the yellowishgreen Tergitol7 agar are indicative of Shigella .
EOSIN METHYLENE BLUE AGAR (LEVINE)
E coli on EMB agar Eosin methylene blue ( EMB also known as ” Levine’s formulation “) is a selective stain for Gramnegative bacteria EMB contains dyes that are toxic to Grampositive bacteria EMB is the selective and differential medium for coliforms It is a blend of two stains eosin and methylene blue in the ratio of 61.
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EMB Agar
The eosin methylene blue agar (EMB agar) is a selective staining for gramnegative bacteria It contains two dyes in the ratio of 61 and is both toxic to grampositive microorganisms It is both a selective and differential mediums for coliforms The color indicator distinguishes microorganisms that are lactose fermenting and nonlactose fermenting.